Translational_Unit

Part:BBa_K415203

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-08-20)


B0031 : cymR (Weak cymR Translation Unit)

This is a translational unit consisting of a weak RBS and the cymR repressor protein; cymR binds tightly and efficiently to the cmt operator, but changes conformation and detaches from DNA when it forms a complex with cumate (isopropybenozate). This part allows for weak translation of mature RNA containing the cymR coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 377
    Illegal XhoI site found at 158
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 60


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Categories
Parameters
None